Other

Part:BBa_K2540011:Design

Designed by: Anna Guseva   Group: iGEM18_Rice   (2018-10-06)


Universal Bacterial Expression Resource (UBER)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 91
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 3912


Design Notes

We have replaced priming promoter with a broad host range one and remove nuclear localization signals to improve UBER from Kushwaha & Salis.


Source

The part was modified from Kushwaha, M. & Salis, H. M. (2015). A portable expression resource for engineering cross-species genetic circuits and pathways. Nature Communications, 6(1), 7832.

References